E-ISSN 2548-0839
European Endodontic Journal Antibacterial Efficacy of the Grape Seed Extract as an Irrigant for Root Canal Preparation []
. 2020; 5(1): 35-39 | DOI: 10.14744/eej.2019.85057

Antibacterial Efficacy of the Grape Seed Extract as an Irrigant for Root Canal Preparation

Fernando Soveral Daviz, Ediléia Lodi, Matheus Alves Albino, Ana Paula Farina, Doglas Cecchin
Department of Restorative Dentistry, Dental School of Universidade de Passo Fundo, Passo Fundo, RS, Brazil.

Objective: The purpose of this research was to compare relative effectiveness of sodium hypochlorite 5.25% (NaOCl), 2% chlorhexidine gel (CHX) and 6.5 % grape seed extract (GSE) against Enterococcus faecalis using instrument Reciproc R25 in root canal preparation.
Methods: Forty-five mesiobuccal root canals from extracted human maxillary molars were collected and infected with Enterococcus faecalis. The samples were divided into five groups according to the different types of irrigants: saline (positive control) (n=5); in the other groups were used 10 root canals for each group: NaOCl+EDTA; CHX gel+EDTA; GSE solution+EDTA; GSE gel+EDTA. All the groups were prepared with reciprocating instruments Reciproc R25. Bacterial reduction was measured by two-way ANOVA (P<0.001) followed by Tukey HSD post-hoc tests, from the counting of colony forming units (CFUs) from samples collected before instrumentation and after. The significance level established at 5% (P<0.05).
Results: The group prepared with the NaOCl resulted in highest antimicrobial capacity among of all (P>0.05), followed by CHX and GSE gel (P<0.05). Control and GSE solution showed similar results (P<0.05) and resulted in the lowest percentage of the reduction of the microorganism into the root canals.
Conclusion: NaOCl had the higher elimination capacity of Enterococcus faecalis than GSE and CHX.

Keywords: Chlorhexidine, enterococcus faecalis, grape seed extract, irrigation, microbiology, reciprocating, sodium hypochlorite

Fernando Soveral Daviz, Ediléia Lodi, Matheus Alves Albino, Ana Paula Farina, Doglas Cecchin. Antibacterial Efficacy of the Grape Seed Extract as an Irrigant for Root Canal Preparation. . 2020; 5(1): 35-39

Corresponding Author: Fernando Soveral Daviz
LookUs & Online Makale