E-ISSN 2548-0839
European Endodontic Journal Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts []
. 2019; 4(2): 57-61 | DOI: 10.14744/eej.2019.74046

Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts

Claudia Meneses, Alessandra Gambini, Lucas Olivi, Marcelo dos Santos, Carla Renata Sipert
Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil

Objective: This study aimed to investigate the cytotoxic and biomodulatory potential of conventional gutta-percha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro.
Methods: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1: 5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P<0.05).
Results: Pure extracts of CGP and CP were found to be cytotoxic for PDL (P<0.01). Once diluted to 1: 5, only CP showed cytotoxicity. BC did not affect cell viability in any extract sample. No extract significantly altered the gene expression of COL-1. For CEMP-1, a significant increase in gene expression was observed only for CGP (P<0.05).
Conclusion: CP was found to be more cytotoxic than CGP, while BC demonstrated no cytotoxicity. The tested cones did not affect COL-1 gene expression, while CGP upregulated CEMP-1. Our results suggest that obturation point components may affect the biological responses of PDL fibroblasts.

Keywords: Bioceramic point, CPoint, cytotoxicity, gutta-percha point, periodontal ligament fibroblasts

Claudia Meneses, Alessandra Gambini, Lucas Olivi, Marcelo dos Santos, Carla Renata Sipert. Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts. . 2019; 4(2): 57-61

Corresponding Author: Carla Renata Sipert

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