E-ISSN 2548-0839
European Endodontic Journal Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material []
. 2018; 3(1): 38-47 | DOI: 10.5152/eej.2017.17018

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Letícia Chaves De Souza1, Mamatha Yadlapati2, Hélio Pereira Lopes4, Renato Silva3, Ariadne Letra3, Carlos Nelson Elias2
1Department of Materials Science Military Institute of Engineering, Rio de Janeiro, RJ, Brazil; School of Dentistry, University of Texas Health Science Center at Houston, TX, USA
2Department of Materials Science Military Institute of Engineering, Rio de Janeiro, RJ, Brazil
3School of Dentistry, University of Texas Health Science Center at Houston, TX, USA
4Department of Endodontics, Estácio de Sá University, Rio de Janeiro, RJ, Brazil

Objective: To propose bismuth carbonate, a radiopacifying agent, as a new endodontic root repair material that was added to Portland cement (PC) at 2 wt%, 5 wt%, 10 wt% and 15 wt%, and physicochemical and biological properties of each formulation were evaluated in comparison to MTA-Angelus.
Methods: Mixed and powder samples were analysed by scanning electron microscopy (SEM) and X-ray power diffraction (XRD), and the semiquantitative constitution of the powder was determined by energy-dispersive spectrometer (EDS). Setting time was evaluated by Vicat needle and radiopacity analysed with digital X-ray. The pH of all tested materials was observed after immersion in water for 3, 24, 48, 72 and 168 h (or 7 days). Solubility and calcium release were measured after immersion in water for 24 h. A multiparametric assay XTT-NR-CVDE was used to evaluate the cytotoxicity of the materials in human periodontal ligament (HPDL) fibroblasts. HPDL fibroblasts were exposed to PC 15% and mineral trioxide aggregate (MTA), and the expression of proinflammatory cytokines (IL1A, IL6, IL8, TNF) and bone formation genes (ALP, COL1, RUNX2) was evaluated by real-time PCR. Mineralisation of HPDL fibroblasts cocultured with PC, PC 15% and MTA was evaluated with Von Kossa staining.
Results: PC-based groups presented more irregular and larger particles than MTA. PC and MTA showed similarities as observed by XRD and EDS. Setting time of PC-based groups was increased with the addition of bismuth carbonate. All tested materials were alkaline, and pH tended to reduce over time. All cements had solubility lower than recommended, with no difference between them (P>0.05) and showed calcium release. PC 15% had similar radiopacity when compared with MTA (P>0.05). Cell viability was higher for the tested materials than the positive control (P<0.001), but there was no difference when they were compared with negative control (P>0.05). Gene expression levels were similar for all tested groups (P>0.05). Analysed cements had positive Von Kossa staining.
Conclusion: Overall, the addition of 15% of bismuth carbonate did not result in significant changes to its physicochemical and biological properties when compared with MTA, except for the setting time, and may be considered a potential substitute for MTA.

Keywords: Bismuth carbonate, mineral trioxide aggregate, portland cement, root repair material

Letícia Chaves De Souza, Mamatha Yadlapati, Hélio Pereira Lopes, Renato Silva, Ariadne Letra, Carlos Nelson Elias. Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material. . 2018; 3(1): 38-47

Corresponding Author: Carlos Nelson Elias, Brazil

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