E-ISSN 2548-0839
European Endodontic Journal Genotoxicity and Hemocompatibility of a Novel Calcium Aluminate-Based Cement (EndoBinder) []
. Ahead of Print: EEJ-43531 | DOI: 10.14744/eej.2018.43531

Genotoxicity and Hemocompatibility of a Novel Calcium Aluminate-Based Cement (EndoBinder)

Rafael Fernández1, Carolina Berruecos1, María Catalina Cortés Motta1, Diego Velásquez2
1Department of Group of Basic and Clinical Sciences in Dentistry, Endodontics, CES University, Medellin, Colombia
2Department of Ingennova Research Group, Biomedical Engineering Research Group EIA – CES (GIBEC), CES University, Medellin, Colombia and EIA University - CES University, Medellín, Colombia

Objective: The aim of this in vitro study was to evaluate the genotoxicity and hemocompatibility of a novel calcium aluminate-based cement, EndoBinder (EB) ( Binderware, São Carlos, SP, Brazil) and compare it to Angelus White Mineral trioxide aggregate (MTA) (AWMTA) (Angelus, Soluções Odontológicas, Londrina, PR, Brazil)–a widely used cement in endodontic microsurgery.
Methods: To determine genotoxicity, a comet assay was performed with Chinese hamster ovary (CHO) cells that had been grown for 24 h in Dulbecco’s Modified Eagle Medium that had been previously incubated with each of the cements for 24 h at 37°C. DNA percentage in head and Olive tail moment were analyzed. For assessment of hemocompatibility, erythrocyte lysis quantification, and concentration of plasma fibrinogen were determined in human blood samples placed in contact with each of the materials. For the hemocompatibility assays, a Student t-test was used to determine the difference between materials. For genotoxicity assays, statistical significance was determined by one-way analysis of variance followed by a Tukey test for multiple comparisons.
Results: Genotoxic effect of EB and AWMTA were comparable to that of the negative control, with no statistically significant differences between AWMTA and negative control (P≥0.05). Compared to AWMTA, EB showed greater hemolytic potential when placed in direct contact with erythrocytes (P≤0.05). Fibrinogen values were low for both materials, with protein concentration being greater in samples exposed to EB than to AWMTA.
Conclusion: Both materials presented a higher hemolytic behavior than what is established by international standards. Fibrinogen formation was low for both materials, and DNA damage induction was not observed in a comet assay.

Keywords: Biocompatibility, blood compatibility, calcium aluminate cement, cytotoxicity, mineral trioxide aggregate



Corresponding Author: Rafael Fernández, Colombia

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