E-ISSN 2548-0839
Antibacterial Effect of Cupral® on Oral Biofilms - An In-Vitro Study []
. 2020; 5(1): 40-45 | DOI: 10.14744/eej.2019.83997

Antibacterial Effect of Cupral® on Oral Biofilms - An In-Vitro Study

Nadine Freifrau Von Maltzahn, Nico Sascha Stumpp
Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover, Germany

Objective: This study aimed to assess the efficacy of Cupral®, a Ca(OH)2 and Cu2+ based materials used in endodontics, against biofilms of the oral species Streptococcus oralis, Streptococcus gordonii and Aggregatibacter actinomycetemcomitans at different maturation stages.
Methods: Biofilms of the bacterial target species were grown in brain heart infusion (BHI) medium for 1 and 5 days on titanium disks (titanium, grade 4) to collect microbial communities at different stages of biofilm maturation. Biofilms were subjected to different Cupral® concentrations (4-, 15- and 50-fold dilution) to assess the antimicrobial- and biofilm dissolving effect. 0.2% chlorhexidine gluconate (CHX) solution was used as a positive control. Biovolume and antibacterial efficacy were analyzed by live/dead staining in combination with confocal laser scanning microscopy (CLSM) to quantify biofilm detachment and antibacterial efficacy.
Results: All tested Cupral® concentration showed a strong antibacterial effect on tested bacterial species at all biofilm maturation stages. Efficacy of biofilms detachment was concentration dependent, i.e. higher Cupral® concentrations generally led to increased biofilm detachment. The antibacterial efficacy of tested Cupral® concentration was at least equal to CHX treatment (P=0.03).
Conclusion: Cupral® shows a strong anti-biofilm efficacy and may be applied for oral biofilm treatment and control in dental disciplines other than endodontics.

Keywords: Antibacterial, antibiofilm, biofilm, biofilm lysis, biofilm detachment, Cupral®, oral infection, treatment

Nadine Freifrau Von Maltzahn, Nico Sascha Stumpp. Antibacterial Effect of Cupral® on Oral Biofilms - An In-Vitro Study. . 2020; 5(1): 40-45

Corresponding Author: Nadine Freifrau Von Maltzahn

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