E-ISSN 2548-0839
A Comparative ex-vivo Study of Effects of Different Irrigation Protocols with/without Laser Activation on the Root Dentine’s Micro-Hardness []
. 2019; 4(3): 127-132 | DOI: 10.14744/eej.2019.49369

A Comparative ex-vivo Study of Effects of Different Irrigation Protocols with/without Laser Activation on the Root Dentine’s Micro-Hardness

Morgana Quteifani Quteifan1, Ahmad Madarati2, Kinda Layous1, Mouhammad Al-tayyan1
1Department of Endodontic and Operative Dentistry, College of Dentistry, Damascus University, Syria
2Department of Retorative Dental Sciences College of Dentistry, Taibah University, Saudi Arabia; Department of Endodontic and Operative Dentistry, College of Dentistry, Aleppo University, Syria

Objective: To compare the effects of different irrigation protocols, with/without laser activation, on the radicular dentine’s micro-hardness.
Methods: Eighty-two human extracted premolars were decoronated and divided into 7 groups. Roots were longitudinally split into two halves. The micro-hardness was measured for one half before and after irrigation protocols. The groups were; G1: MTAD without laser-activation, G2: MTAD with laser-activation, G3: sodium-hypochlorite (SH) with laser-activation, G4: SH then EDTA with laser-activation, G5: SH then MTAD with laser-activation, G6: SH without laser-activation. G7: distilled water (control). In the two-irrigants groups G4 and G5), samples were irrigated first with SH then with MTAD or EDTA irrigants, which were activated by the laser. The difference between the before- and after-irrigation micro-hardness was calculated to obtain the micro-hardness difference. Data were analyzed using the Paired Sample-t and Two-ways ANOVA tests at P=0.05.
Results: Overall, the mean dentine’s micro-hardness after-irrigation (103.1) was lower than before-irrigation (116.1) (P<0.001); except for the distilled-water group, (116.6 and 112.9, respectively) (P=0.075). The micro-hardness reduction of SH without laser-activation group (32.5) was the greatest (P<0.001). The single-irrigant or laser-activation irrigation protocols caused significantly less micro-hardness reduction compared to the two-irrigants or no laser-activation protocols. The mean micro-hardness reduction of SH and MTAD groups (both with laser-activation) (5.8 and 9.3, respectively) were significantly lower than other groups, but not from that of the control group (3.7).
Conclusion: Using irrigants significantly reduced the root-dentine’s micro-hardness. Although irrigants agitation by an Er: Yag laser significantly minimized micro-hardness reduction, it did not suppress the adverse effects on dentine micro-hardness when two-irrigants were used.

Keywords: Agitation, EDTA, irrigation, laser, micro-hardness, MTAD, sodium hypochlorite

Morgana Quteifani Quteifan, Ahmad Madarati, Kinda Layous, Mouhammad Al-tayyan. A Comparative ex-vivo Study of Effects of Different Irrigation Protocols with/without Laser Activation on the Root Dentine’s Micro-Hardness. . 2019; 4(3): 127-132

Corresponding Author: Ahmad Madarati

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