E-ISSN 2548-0839
European Endodontic Journal Cytotoxicity and Genotoxicity of Calcium Hydroxide and Two Antibiotic Pastes on Human Stem Cells of The Apical Papilla [Eur Endod J]
Eur Endod J. 2021; 6(3): 303-308 | DOI: 10.14744/eej.2021.97658

Cytotoxicity and Genotoxicity of Calcium Hydroxide and Two Antibiotic Pastes on Human Stem Cells of The Apical Papilla

Davood Jamshidi1, Mohamadreza Ansari2, Nematollah Gheibi3
1Department of Endodontics, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran; Department of Endodontics, Faculty of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran
2Department of Endodontics, Faculty of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
3Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

Objective: This study aimed to assess the cytotoxicity and genotoxicity of triple antibiotic paste, double antibiotic paste and calcium hydroxide medicaments on human stem cells of the apical papilla.
Methods: In this experimental study, stem cells were isolated from the apical papilla and cultured. They are treated with different concentrations 0.1, 0.5, 1, 10 and 100 mg/ml of medicaments for 24, 48 and 72 hours. The cytotoxicity and genotoxicity of the medicaments were determined using methyl thiazolyl tetrazolium assay and Comet test, respectively.
Results: Results showed all tested concentrations of the calcium hydroxide had no significant effect on stem cells at any time point. Triple antibiotic paste showed cytotoxicity in 10 and 100 mg/mL concentrations at all-time points and in 1, 10 and 100 mg/ml concentrations at 72 hours. In addition, its genotoxicity was significantly higher than that of other groups (P<0.05). Double antibiotic paste showed cytotoxic effects only in 100 mg/ml concentration at 24 hours and 10 and 100 mg/ml concentrations at 48 and 72 hours. And also, its genotoxicity in these concentrations was significantly higher than that of control and calcium hydroxide groups (P<0.05).
Conclusion: In contrast to calcium hydroxide, triple antibiotic paste and double antibiotic paste, especially in their higher concentrations, induced cytotoxicity and genotoxicity on human stem cells of the apical papilla. (EEJ-2020-12-289)

Keywords: Calcium hydroxide, cytotoxicity, double antibiotic paste, genotoxicity, triple antibiotic paste

Davood Jamshidi, Mohamadreza Ansari, Nematollah Gheibi. Cytotoxicity and Genotoxicity of Calcium Hydroxide and Two Antibiotic Pastes on Human Stem Cells of The Apical Papilla. Eur Endod J. 2021; 6(3): 303-308

Corresponding Author: Mohamadreza Ansari
Manuscript Language: English
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