Effectiveness of Three Rotary File Systems on Enterococcus faecalis in Single Rooted Teeth: An In Vitro Study

Objective: To evaluate the effectiveness of three rotary file systems on the elimination of Enterococcus faecalis (E. faecalis) biofilms in single-rooted teeth.
Methods: A total of 70 mandibular premolars were investigated. N=60 of the root canals were inoculated with E. faecalis. The positive control group (n=5) received bacterial inoculation without mechanical preparation. The negative control group (n=5) was neither infected nor mechanically prepared. Teeth were randomly assigned to 3 experimental groups (n = 20 per-group) according to application of the rotary files: Group 1: XP-endo Rise Shaper (FKG, Le Crêt-du-Locle, Switzerland); Group 2: XP-endo Shaper (FKG, Le Crêt-du-Locle, Switzerland); Group 3: ProTaper Gold (Dentsply Sirona, Erlangen, Germany). Samples were collected using paper points before (S1) and after (S2) mechanical preparation. Colony-forming units (CFUs) were used to quantify bacterial loads.
Results: Significant differences in bacterial counts were observed from S1 to S2 in all tested groups (p < 0.001). Intergroup comparisons revealed no significant differences between the XP-endo Rise Shaper and XP-endo Shaper groups (p > 0.05). nor XP-endo Shaper and ProTaper Gold groups (p > 0.05). Significant differences were observed between the XP-endo Rise Shaper and ProTaper Gold groups (P<0.05). The XP-endo Rise shaper group showed the largest reduction in bacterial loads (98.2%), followed by the XP-endo Shaper (96.0%) and ProTaper Gold (93.8%) groups.
Conclusion: All the rotary files in our study were able to reduce bacteria but the novel single-file
system (XP-endo Rise shaper) was superior in reducing bacterial load compared to XP-endo
Shaper and the multiple file system (ProTaper Gold). (EEJ-2024-08-133)