Eur Endod J. Ahead of Print: EEJ-36450 | DOI: 10.14744/eej.2024.36450

Effectiveness of Laser-activated Irrigation Modalities on Intracanal Bacterial Elimination and Apical Extrusion

Mohamed Marwan Abbas¹, Sunaina Shetty Yadadi¹, Ashna Sulaiman², Okba Mahmoud³, Ahmed Jamleh¹, Saaid Al Shehadat^1
1Department of Restorative Dentistry, University of Sharjah, College of Dental Medicine, Sharjah, United Arab Emirates
²University of Sharjah, Research Institute for Medical and Health Sciences, Sharjah, United Arab Emirates
³Department of Clinical Sciences, Ajman University, Faculty of Dentistry, Ajman, United Arab Emirates

Objective This study aimed to compare photon-induced photoacoustic streaming (PIPS) and diode laser with passive ultrasonic irrigation (PUI) in activating intracanal irrigants for bacterial elimination from the root canal and apical extrusion.
Methods: Sixty extracted single-canal human teeth were chemo-mechanically prepared and placed in 3ml glass vials with sterile 0.9% sodium chloride solution. The root canals were inoculated with Enterococcus faecalis and incubated for 24 hours at 37°C. The samples were divided into three experimental groups (PIPS, diode laser, and PUI) and a control group (n=15 each). The experimental groups had root canals filled with saline and activated according to manufacturer instructions, while the control group received saline without activation. Bacterial samples were collected from the canals and outside the apex for quantification, cultured on nutrient agar for 24 hours at 37°C, and counted as colony-forming units. Mean values were compared using one-way ANOVA and Bonferroni tests at 5% significance.
Results All activation protocols significantly eliminated intracanal E. faecalis compared to the negative control group (p<0.05). PUI and the diode laser were significantly more effective than PIPS (p<0.05). Extruded bacteria were higher in PUI than PIPS and diode laser.
Conclusion: Within the limitations of this study, the tested techniques extruded bacteria and did not completely eliminate intracanal bacteria. The diode laser showed the best outcome in terms of bacterial elimination and extrusion. (EEJ-2024-07-112)

Keywords: Diode laser, laser activated irrigation, passive ultrasonic irrigation, photon-induced photoacoustic streaming, root canal disinfection

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