Eur Endod J. 2023; 8(4): 280-285 | DOI: 10.14744/eej.2023.59672

The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells

Valonia Irene Nugraheni¹, Dini Asrianti Bagio¹, Anggraini Margono¹, Indah Julianto^2
1Department of Conservative Dentistry, Universitas Indonesia Faculty of Dentistry, Jakarta, Indonesia
²Department of Dermatology and Venereology, Universitas Sebelas Maret Faculty of Medicine, Surakarta, Indonesia

Objective: Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix. In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and differentiate into odontoblast like cell, creating reparative dentine and expressing dentine sialophosphoprotein (DSPP). Therefore, the purpose of this study was to analyze the potential of hydrogel HA in various concentration towards hDPSCs differentiation via DSPP expression at day 7 and 14.
Methods: After hDPSCs incubation reaching 80% confluence, cells were then starved for 24 hours. Then, culture media were supplemented with osteogenic media. hDPSCs planted into 96 well plate and HA 10 μg/mL, 20 μg/mL, and 30 μg/mL were added. DSPP expression was analysed using elisa reader at day 7 and 14, qualitative result was analysed using alizarin red at day 21. Data was analysed using one-way ANOVA.
Results: At day 7, there was a statistically significant different potential of HA conditioned media in various concentration (p<0.05) towards hDPSCs differentiation via expression of DSPP with HA 30 μg/mL being the most potential concentration to increase DSPP expression.
Conclusion: HA have the potential to increase odontoblast differentiation process via expression of DSPP, with HA 30 μg/mL being the optimum concentration for hDPSCs. (EEJ-2022-12-169)

Keywords: Culture media, dental pulp, dentine sialophosphoprotein, hyaluronic acid, regenerative endodontics, stem cells, tissue regeneration

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