E-ISSN 2548-0839
Volume : 9 Issue : 1 Year : 2024

Metrics

1.8
2022 IMPACT FACTOR
1.6
5 year Impact Factor
0.00041
Eigenfactor
2.6
2022 CiteScore
90/157
Journal Citation Reports (Clarivate, 2023)(Dentistry, Oral Surgery & Medicine (Science))
SCImago Journal & Country Rank
Evaluation of Bacterial Reduction at Various Stages of Endodontic Retreatment After Use of Different Disinfection Regimens: An In Vivo Study [Eur Endod J]
Eur Endod J. 2022; 7(3): 210-216 | DOI: 10.14744/eej.2022.42713

Evaluation of Bacterial Reduction at Various Stages of Endodontic Retreatment After Use of Different Disinfection Regimens: An In Vivo Study

Jaynit Tandon1, Sonali Taneja1, Vidhi Kiran Bhalla1, Anshi Jain2
1Department of Conservative Dentistry and Endodontics, ITS Centre for dental studies and Research, Uttar Pradesh, India
2Department of Oral Pathology, ITS centre for dental studies and Research, Uttar Pradesh, India

Objective: The present study was conducted to evaluate the presence of aerobic bacteria, anaerobic bacteria, E. faecalis, F. nucleatum, Propionibacteria sp., Actinomyces sp., and their reduction at various stages of endodontic retreatment with the use of conventional protocol (5.25 % Sodium Hypochlorite (NaOCl) as the irrigant along with Calcium Hydroxide (Ca (OH)2) as intracanal medicament and advocated protocol (SmearOFF as the irrigant along with 2% Chlorhexidine (CHX) gel as intracanal medicament).
Methods: Twenty eight patients fulfilling the eligibility criteria were selected for root canal retreatment and randomly allocated into two groups. Group 1: Final irrigant as SmearOFF+Chlorhexidine 2% gelas intracanal medicament (n=14). Group 2: Final irrigant as 5.25% NaOCl+Ca(OH)2 as intracanal medicament (n=14). With aseptic environment, access opening was performed followed by Gutta Percha (GP) removal and sample S1 was collected for bacterial analysis. The biomechanical preparation was done by using Reciproc system with additional finishing with XP-Endo Finisher R. Sample S2 was then collected for bacterial analysis after the final irrigation protocol in the respective groups. Intracanal medicaments were placed for one week and sample S3 was collected. All the samples were subjected to qualitative analysis using PCR and quantification was done by Colony Forming Unit (CFU) analysis.
Results: Aerobic [28/28], Anaerobic [28/28], Propionibacterium sp. [20/28] and F. nucleatum [24/28] were the most frequently isolated in S1 sample followed by Actinomyces sp. [16/28] and E. faecalis sp. [19/28]. Chemico-mechanical preparation followed by irrigation (S2 sample) resulted in significant reduction of all types of bacteria in both groups. Group-1 (SmearOFF as the final irrigant) had significantly superior efficacy against aerobic bacteria, E. faecalis and F. nucleatum (P<0.05) as compared to Group-2 (NaOCl). After medicament placement, significant differences between the groups were noted only for the E. Faecalis group. For the S3 samples, the mean bacterial reduction was significant in Aerobic and F. nucleatum in S3 samples for Group 1 and Group 2.
Conclusion: Chemico-mechanical preparation followed by irrigation resulted in significant reduction in bacterial load irrespective of the final irrigant. SmearOFF was significantly better than NaOCl in minimizing bacterial load of E. faecalis and F. nucleatum. 2% Chlorhexidine gel has superior antimicrobial efficacy against E. faecalis and may be recommended in secondary endodontic treatment. (EEJ-2022-01-05)

Keywords: Chlorhexidine, endodontic microbiology, endodontic retreatment, SmearOFF

Jaynit Tandon, Sonali Taneja, Vidhi Kiran Bhalla, Anshi Jain. Evaluation of Bacterial Reduction at Various Stages of Endodontic Retreatment After Use of Different Disinfection Regimens: An In Vivo Study. Eur Endod J. 2022; 7(3): 210-216

Corresponding Author: Sonali Taneja
Manuscript Language: English
LookUs & Online Makale