E-ISSN 2548-0839
Evaluation of the Cytotoxicity and Apoptotic Effects of Nano Triple Antibiotic Paste with Nano Anti-Inflammatory Drug as an Intracanal Medicament [Eur Endod J]
Eur Endod J. 2021; 6(1): 82-89 | DOI: 10.14744/eej.2020.29292

Evaluation of the Cytotoxicity and Apoptotic Effects of Nano Triple Antibiotic Paste with Nano Anti-Inflammatory Drug as an Intracanal Medicament

Yousra Nashaat1, Hadil Sabry2, Soha Ahmed Hassan3
1Department of Endodontic, Faculty of Dentistry, University of October 6, Cairo, Egypt
2Department of Dental Biomaterials, Faculty of Dentistry, University of October 6, Cairo, Egypt
3Department of Histology, Faculty of Dentistry, University of October 6, Cairo, Egypt

Objective: The aim of this study is to compare the cytotoxicity of triple antibiotic paste (TAP) with an anti-inflammatory drug (TAP+Catafast-TAPC) in nano and regular formulations versus calcium hydroxide as intracanal medicaments.
Methods: The TAPC drugs extraction were made in cell culture media MEM-E (Eagle's minimal essential medium) using concentration of 10 mg/mL of each sample for seven days. Inhibitory concentrations (IC50 values) were determined for each extract. A human fibroblasts cell line was used to evaluate the cytotoxicity of different concentrations (10, 0.625 and 0.07 mg/mL) using MTT essay. The cell viability was measured after 24 h, 48 h and 7 days for all concentrations of the drugs. Flow cytometry analysis was carried out to identify the effect of materials on apoptosis/necrosis. Statistical analysis for the obtained results was done by one-way ANOVA.
Results: The results revealed that cell viability was inversely proportional to the duration of treatment in all of the groups. Calcium hydroxide (Control group) demonstrated a significantly greater cytotoxic effect, followed by Nano Triple Antibiotic Paste with Catafast as an anti-inflamatory drug (Nano TAPC), while Triple Antibiotic Paste with Catafast (TAPC) had the least cytotoxic effect. Nano TAPC has the greatest apoptotic value, while TAPC had the least when compared with the reference group, with no significant difference between groups (P<0.05).
Conclusion: The cytotoxic effect of Nano TAPC was lower than that of calcium hydroxide and higher than that of TAPC. Although Nano TAPC has the highest apoptotic value when compared to TAPC and calcium hydroxide but still there is no statistically significant difference between them. (EEJ-2020-09-222)

Keywords: Apoptosis, cytotoxicity, flow cytometry, MTT, Nano TAPC, Root canal

Yousra Nashaat, Hadil Sabry, Soha Ahmed Hassan. Evaluation of the Cytotoxicity and Apoptotic Effects of Nano Triple Antibiotic Paste with Nano Anti-Inflammatory Drug as an Intracanal Medicament. Eur Endod J. 2021; 6(1): 82-89

Corresponding Author: Yousra Nashaat
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